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KMID : 1094019940110010389
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Journal of Veterinary Clinics
1994 Volume.11 No. 1 p.389 ~ p.392
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Study on the Purification of Transforming Growth Factor-$\beta$ in Canine Platelets
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Abstract
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To purify transforming growth factor type beta(TGF-β) in canine platelets, Sephadex G-75 gel filtration and semipreparative HPLC were carried out. The column of 2.0×120cm was used for gel filtration and one inch semipreparative column filled with SP-Toyopeal for HPLC. Electrophoresis and bioassay using African green monkey kidney cell were used for identification of TGF-β Crude TGF-β of 2.75mg was extracted from 5.2g of the platelets by the treatment of acid/ethanol. In gel filtration of crude TGF-β, 4 peaks were observed at the detection of spectrophotometer at 280nm. Electrophoresis and bioassay identified the 3rd peak TGF-β. Linear gradient elution from 0 to 3M NaCl in sornipreparative HPLC showed TGF-β at 1.5M NaCl. Gel filtration was less expensive and useful method for the purification of TGF-β.
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